Singleplex and Multiplex Bead Based Immunoassays
Meso Scale Discovery V-PLEX HUMAN TNF-A KIT
Human tumor necrosis factor alpha (TNF-), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2) and cachectin, is a 25.6 kDa cytokine. TNF is a transmembrane protein that oligomerizes intracellularly to form a non-covalent homotrimer. The membrane-bound soluble portion of the homotrimer is cleaved by TACE/ADAM17 to form TNF-. The homotrimer binds to the receptors TNF RI and TNF RII, both of which are also expressed as homotrimers. TNF- is mainly secreted by macrophages and can induce apoptosis in some tumor cell lines. It stimulates IL-1, which induces cachexia and causes fever. The intracellular form of TNF induces IL-12 production in dendritic cells. It can induce sepsis and inflammation and can inhibit tumorigenesis and viral replication. The Human TNF- Kit provides assay-specific components for the quantitative determination of endogenous TNF- in human urine, serum, plasma, and cell culture supernatants.
Meso Scale Discovery UPLEX MSE MCP1 ASY384well
The Meso Scale Discovery U-PLEX Mouse MCP-1 384-well Assay Kit is a multiplex immunoassay that measures mouse monocyte chemoattractant protein 1 (MCP-1) in serum plasma and cell culture supernatants The kit uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision It requires just 25 L of sample per well and the 384-well plate allows high-throughput analysis The assay takes approximately 3 5 hours to complete
Cayman Chemical THUNDERHumn IL8 TRFRET B 96Wel
Sandwich TR-FRET immunoassay for the quantitative measurement of human IL-8 (CXCL8; CGP-1; NAP-1) in cell culture supernatants
Meso Scale Discovery UPLEX HUM TGFB2 ASYQP 25 PL
The Meso Scale Discovery U-PLEX Human TGF-beta2 Assay Kit is designed for the quantitative measurement of active and total TGF-beta2 in human serum plasma and cell culture supernatants using an electrochemiluminescent detection method The assay uses MSD s proprietary QuickPlex SQ 120 instrument and provides high sensitivity and a broad dynamic range This multiplex kit includes a plate coated with capture antibodies detection antibodies and calibrators and controls needed to generate a standard curve and quantify sample concentrations The assay has high specificity for active and total TGF-beta2 with minimal cross-reactivity to related analytes
Biolegend LEGENDplex™ Mouse IL-17F Capture Bead B5, 13X
LEGENDplex™ Mouse IL-17F Capture Bead B5, 13X Apps: Multiplex - Component; Size: 100 tests (270 uL)
ABclonal Technology CCL7 Rabbit pAb
This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q.
Cayman Chemical SemiQuantitative Biomarker EL
This assay contains a set of reagents to be used together with a suitable Biosense primary antibody for semi-quantitative detection of biomarkers such as Vtg, CYP1A, Zrp, and MT in samples from fish.
Biolegend LEGENDplex™ Human MMP-2 Capture Bead A7, 13X; Reactivity: Human; Apps: Multiplex - Component; Size: 100 tests (270 μL)
LEGENDplex™ Human MMP-2 Capture Bead A7, 13X; Reactivity: Human; Apps: Multiplex - Component; Size: 100 tests (270 μL)
Meso Scale Discovery UPLEX HUM TGFB1 ASY384well
The Meso Scale Discovery U-PLEX Human TGF-beta1 384-well Assay Kit is a multiplex immunoassay that measures TGF-beta1 levels in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and rapid results This 384-well plate assay allows for high throughput screening and only requires 10-25 L sample volume per well The kit includes a 384-well MULTI-ARRAY Plate pre-coated with capture antibodies detection antibodies read buffer calibrators and controls needed to quantify TGF-beta1 levels It has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL
Meso Scale Discovery UPLEX MSE MIP1 ASYQP
The U-PLEX Gateway assay kits from Meso Scale Discovery enable researchers to create custom multiplex immunoassays for simultaneous measurement of multiple biomarkers in a single sample These electrochemiluminescence-based assays offer superior sensitivity wide dynamic range and reduced sample volume requirements compared to traditional methods The platform supports flexible assay development with pre-validated antibody pairs and allows detection of up to 10 analytes per well making it ideal for biomarker discovery drug development and clinical research applications
Biolegend LEGENDplex™ MU Cytokine Release Syndrome Panel Standard
LEGENDplex™ MU Cytokine Release Syndrome Panel Standard Reactivity: Mouse; Apps: Multiplex - Component; Size: 1 vial
Meso Scale Discovery U-PLEX Human CTLA-4 ASYQP
The Meso Scale Discovery U-PLEX Human CTLA-4 Assay Kit is a multiplex immunoassay for measuring CTLA-4 in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection for high sensitivity and precision The assay requires 50 L sample volume and takes 3 5 hours It includes a 96-well plate with capture antibodies detection antibodies with MSD SULFO-TAG Read Buffer Wash Buffer Blocker A and calibrators It tests up to 80 samples in duplicate with a detection limit of 0 056 ng/mL
Meso Scale Discovery U-PLEX Human Ghrelin (total) Assay, SECTOR (1 PL)
The U-PLEX platform offers validated components that can be combined to create high quality assays quickly and easily. U-PLEX Assay has the essential components for an assay that measures single-analyte format. For information on multiplexing with other analytes, please see U-PLEX Custom Metabolic Group 1 (human) Assays. Related Key Terms:Glucagon-Like Peptide-1, GLP-1, metabolic diseases, metabolism, proglucagon, insulin, diabetes